Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. Ficoll and dextran vs. globular proteins as probes for testing Careers. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. (1969). Dextran. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. The above data are not useful because it can not be compared with those obtained in this work. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was What is hydrodynamic radius In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. Dextrans in Aqueous Solution. Experimental Review on Intrinsic The enhanced permeability through micropores was confirmed by Franz diffusion cell. FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). 2011;5(2):95132. Journal of Chemical Education, 49(8), 577-579. Walkenhorst, S.; Olivier, R. (2002). what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. A single frequency laser is directed to the sample contained in a cuvette. Webdextran hydrodynamic radius. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). statement and (2008). Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. B 113, 8020-8025. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free (1987). Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. 2012;87(6):1728. Physicochemical studies of oligodextran. The site is secure. Huggins, Maurice L. (1942). Small Intestinal Permeability and Gut-Transit Time Determined Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. 2012;9(2):14754. Carbohydrate Polymers 88 373-381. This result could reflect the presence of some aggregated species within the lysozyme sample solution. Biomaterials Research DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). Journal of Colloid Science, 31, 308-328. Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. Kasaai, Mohammad R. (2007). Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. Drug Deliv. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. The Mark-Houwink. Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. MeSH GPC Symp. Synthesis and characterization of blue dextrans. Cell permeabilization using a non-thermal plasma - IOPscience (2006). Nat Biotechnol. Analytical Chemistry. Unable to load your collection due to an error, Unable to load your delegates due to an error. Global hydrodynamic analysis of the molecular flexibility of galactomannans. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). Learn More. Dextran Guner, A.; Kibarer, G. (2001). The micropore formed at the SC through to the epidermis, enabling drug delivery. Solvent effects on polysaccharide conformation. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. volume24, Articlenumber:22 (2020) Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Learn More. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. Stokes radius Table 2 shows that comparing the intrinsic viscosity data obtained from the Huggins method and the Solomon-Ciuta single-point method, a similar values are obtained and the %RE is lower for low Mw and it increases for higher Mw. Epub 2017 Jun 1. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. ; Weisz, J. ; Fran, H.P. 2009;301(8):60913. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. Federal government websites often end in .gov or .mil. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Accessibility ; Aberle, T; Burchard, W. (2000). The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. For data requests, please contact the authors. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. Carbohydr Polym. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). Polysaccharide hydrogels for modified release formulations. These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28]. Principles of Size Exclusion Chromatography SEC (GFC The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Hydrodynamic radii ( RH) were determined by dynamic light scattering. Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. Treatment of acne scars by fractional bipolar radiofrequency energy. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. (2007). Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively. dextran hydrodynamic radius. Weissella: An Emerging Bacterium with Promising Health Benefits. The skin poration was performed using the microporation device (LG electronics, Korea). 8600 Rockville Pike The term f/f0 is sometimes denoted as P, Perrin constant. Cite this article. Expert Rev Med Devices. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. Charge: Anionic. Hydrodynamic Radius In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. Influence of molecular weight on Probiotics Antimicrob Proteins. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. Ahn, G.Y., Eo, HS., Kim, D. et al. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). Arch Dermatol Res. All authors have consented to the submission of this manuscript for publication. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. Masuelli, Martin Alberto (2013). Garcia de la Torre, J.; Carrasco, B. government site. Kasaai, Mohammad R. (2012). WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). In fact, short chains do contain minor branching points. 2014 Sep;5(9):2324-30. doi: 10.1039/c4fo00319e. A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. 4D imaging reveals mechanisms of clay-carbon protection and The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). The Mark-Houwink a constant is close to 0.5 in poor solvents. Colloid Polym. ; van Nostrum, C.F. and transmitted securely. FITC-dextran solution was prepared at 1mM in PBS. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. Chem. Dextran Fractions. Food Sci Biotechnol. Expert Opin Drug Deliv. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. What is dextran? Application areas - TdB Labs AB The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. Human skins (Full-thickness human skin) were supplied by HansBiomed. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. We observe that this maximum radius is below Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish II. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. Molecular dynamics simulations on aqueous Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. Anal. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. PMC WebR H Hydrodynamic radius [m] The basic DLS setup. Microporation techniques for enhanced delivery of therapeutic agents. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. He, L.; Niemeyer, B. In addition, we evaluated the regeneration of SC after microporation. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with ; Ghosh, T.K. Skin barrier disruption by acetone: observations in a hairless mouse skin model. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. To confirm the extent of skin damage and regeneration after microporation, porations were performed 2, 5, and 10 times. This site needs JavaScript to work properly. Ioan, C.E. Dextrans can function efficiently as anterograde or retrograde tracers, Whiteside PJD, Chininis JA, Schellenberg MW, Qian C, Hunt HK. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. ; Garca de , J. J Cosmet Laser Ther. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. AATEX.